Ethidiumbromid 0,625 mg/l i vandigopløsning, Biotechnology Grade

Leverandør: VWR Chemicals
Ratings: Ingen bedømmelse

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Synonymer: 3,8-Diamino-5-ethyl-6-phenylphenantridiniumbromid

E406-5MLEA 231 DKK
Ethidiumbromid 0,625 mg/l i vandigopløsning, Biotechnology Grade

Ethidium bromide solution is a fluorescent stain for visualising nucleic acids in solution or in electrophoresis gels. The solution is provided at a concentration of 0,625 mg/ml. A final working concentration of 0,5 g/ml is easily obtained by adding one drop of solution to 50 ml of molten agarose, running buffer or staining buffer. Ethidium bromide solution in dropper bottles may be stored at room temperature for up to one year.

  • Used to detect as little as 10 ng of nucleic acid
  • Dropper bottles are shatter-resistant and light-proof
  • No additional hazardous shipping costs are applied to ethidium bromide dropper bottles

At a concentration of 0,5 μg/ml, ethidium bromide is useful in agarose or acrylamide gels for band detection.

At higher concentrations (0,5 to 1,0 μg/ml), it may be used to facilitate purification of DNA in a caesium chloride gradient.

One drop diluted into 50 ml of molten agarose or buffer yields a final concentration of 0,5 μg/ml.

Emballering: In 0,625 mg/ml dropper bottles.

Formel: C₂₁H₂₀BrN₃
Molekylvægt: 394,31 g/mol
MDL Number: MFCD00011724
CAS-nummer: 1239-45-8
EINECS: 214-984-6

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Specifikationer Testresultater

Abs.@ Lambda Max 0.68 – 0.74
DNase none detected
Lambda Max (8/100 ml Water) 478 – 482 nm
RNase none detected

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