Ready-to-Load™ kits, in search of the sickle cell gene by southern blotLeverandør: Edvotek
Ratings: Ingen bedømmelse
EDVO315EA 1930 DKKEDVO315
Ready-to-Load™ kits, in search of the sickle cell gene by southern blot
Undervisningsmateriale Biology Educational Materials Genetics Learning Activities
Southern blotting is an important technique used widely in clinical genetics and research. By transferring DNA from an agarose gel onto a membrane the method allows the precise analysis and identification of DNA bands on a gel. Using this kit, students use Southern blotting to find a point mutation in the haemoglobin gene indicating Sickle Cell Anaemia.
- For 5 lab groups
- Electrophoresis, 45 minutes; blotting, overnight; staining and destaining, 10 minutes
Also required: Electrophoresis tank, power supply, water bath and 80 °C incubator.
Leveringsinformation: Kit includes instructions, Ready-to-Load™ DNA samples, agarose, electrophoresis buffer, nylon membranes, filter paper, and blotting stain.