Transformation with green fluorescent protein

Leverandør: Edvotek
Ratings: Ingen bedømmelse

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EDVO223/AP08EA 1120 DKK
Transformation with green fluorescent protein
Undervisningsmateriale Biology Educational Materials Microbiology Learning Activities
In this experiment, transformed cells take up a plasmid containing the Green Fluorescent Protein (GFP) gene. The GFP gene was isolated from the jellyfish Aequorea victoria. Transformed colonies expressing the GFP protein are visibly green in normal light but will fluoresce brightly when exposed to longwave UV light.

  • For 10 lab groups
  • Plating 5 minutes; incubation overnight; transformation efficiency 15 minutes

Also required: 37 °C incubator, water bath, longwave UV lamp.

Leveringsinformation: Kit includes instructions, cells, plasmid DNA, IPTG, ampicillin, transformation solution, ReadyPour agar, Luria broth, petri dishes, sterile pipettes, loops and microtubes.
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