Experimentation kits, cleavage of Lambda DNA with Eco RI endonuclease: intro to restriction enzymes

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EDVO212EA 1450 DKK
EDVO212
Experimentation kits, cleavage of Lambda DNA with Eco RI endonuclease: intro to restriction enzymes
Undervisningsmateriale Biologisk undervisningsmateriale Biochemistry Learning Activities
The DNA from bacteriophage Lambda is a well-characterised linear molecule containing six recognition sites for Eco RI (5 distinct sites; 2 are very close in size). In this experiment, Lambda DNA is digested by the Eco RI endonuclease. The digestion products are analysed by agarose gel electrophoresis.

  • For 5 lab groups
  • Complete in 90 minutes

Also required: Electrophoresis tank, power supply, 5 to 50 µl and 40 µl fixed volume micropipettes, water bath, and white light box.

Leveringsinformation: Kit includes instructions, Lambda DNA, Dryzymes™, reconstitution buffer, restriction enzyme reaction buffer, enzyme grade water, standard DNA fragments, various solutions and buffers, agarose powder, and stains.
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