Tissue freezing medium, PolyFreeze, Sigma-Aldrich®Leverandør: Merck
- Colored PolyFreeze (blue, green, yellow) makes differentiating multiple specimens easy during sectioning and staining
- Experience less curling, less ice artifacts, faster freezing with PolyFreeze
um are easy to obtain and mount on glass slides for drying and/or fixation prior to staining, while thicker sections can be taken as required. Sections will flow freely under an anti-roll device, allowing flat sections to be picked up from the knife-edge. Sections can be either fixed immediately in the fixative of choice or air dried for later fixation and staining. Drying or fixing the slides in the cryostat for specific procedures will not affect the PolyFreeze medium.
Cryo-embedding matrix for frozen specimens.
PolyFreeze tissue freezing medium has been used in immunohistochemistry.
A support matrix or form of embedding medium for frozen sectioning. This medium freezes quickly supporting the tissue for sectioning at 3 μ and up with no cracking of the matrix at temperatures from -8 °C to -25 °C. Snap freeze using PolyFreeze with isopentane and liquid nitrogen or dry ice (slush/slurry or bunker). Store specimens frozen in PolyFreeze in liquid nitrogen canisters or in airtight containers in a -80 °C freezer.
Store specimens frozen in PolyFreeze medium in liquid nitrogen canisters or in airtight containers in a -80 0C freezer.