Anti-citrullinated human fibrinogen assay kit (Mouse)

Leverandør: Cayman Chemical
Ratings: Ingen bedømmelse

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CAYM501270-96EA 13670 DKK
CAYM501270-96
Anti-citrullinated human fibrinogen assay kit (Mouse)
Assays ELISA'er
For measuring citrullinated fibrinogen antibodies in mouse plasma or serum.

  • Measure antibodies specific for citrullinated human fibrinogen
  • Use with mouse plasma or serum
  • Human fibrinogen affinity sorbent included for removal of any antibodies capable of reacting with non-citrullinated (unmodified) fibrinogen prior to analysis

Immunization of mice with citrullinated human fibrinogen, especially mice expressing the human HLA-DR4 transgene, induces an arthritic response driven by the production of antibodies that recognize citrullinated epitopes. The polyclonal antibody response produces antibodies reactive with both citrullinated human fibrinogen and unmodified, non-citrullinated human fibrinogen. Cayman’s Anti-Citrullinated Human Fibrinogen Assay (mouse) is an immunometric (sandwich) assay that can be used to distinguish the antibody response to citrullinated human fibrinogen from the antibody response to unmodified human fibrinogen in mouse serum or plasma. A human fibrinogen affinity sorbent is provided with the kit so that any antibodies capable of reacting with non-citrullinated (unmodified) fibrinogen can be removed prior to analysis of the remaining anti-citrullinated fibrinogen antibodies for an accurate analysis of the anti-citrulline response. This kit uses a citrullinated human fibrinogen-coated plate and a detection antibody recognising mouse IgG. An anti-citrullinated human fibrinogen monoclonal antibody is included as a standard. The standard curve spans the range of 0,15 to 10 µg/ml, with an an LLOQ of 0,15 µg/ml.

Leveringsinformation: Kit contains plate and cover sheet, affinity sorbent, conjugate, immunoassay buffer B and wash buffer concentrate, polysorbate 20, substrate and stop solution.
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