A competitive assay that can be used for quantification of 2-methoxyestradiol in plasma, urine, and other sample matrices.
- Detect 2-ME2
- Assay 24 samples in triplicate or 36 samples in duplicate
- Measure 2-ME2 levels down to 40 pg/ml
- Colorimetric read at 405 to 420 nm
2-Methoxyestradiol (2-ME2) is a natural metabolite of estradiol produced by CYP450-dependant hydroxylation followed by catechol-O-methyltransferase-catalyzed O-methylation. Deficiency in catechol-O-methyltransferase is associated with pre-eclampsia. Therefore, 2-ME2 may be utilized as a plasma and urinary diagnostic marker for this disease, as well as a therapeutic supplement for its prevention. Several clinical trials have demonstrated the antitumor effects of 2-ME2 in prostate cancer, breast cancer, renal cell carcinoma, ovarian cancer, glioblastoma, and multiple myeloma. Other studies indicate that 2-ME2 exhibits more potent cardioprotective effects than estradiol. Cayman’s 2-Methoxyestradiol ELISA Kit is a competitive assay that can be used for quantification of 2-ME2 in plasma, urine, and other sample matrices.
Leveringsinformation: Kit contains antibody, tracer, standard, ELISA and wash buffer concentrate, polysorbate 20, plate and cover sheet, Ellman's reagent, tracer and antiserum dye.