The Glucose (HK) Assay Kit is for the quantitative, enzymatic determination of glucose in food and other material.
Glucose is phosphorylated by adenosine triphosphate (ATP) in the reaction catalysed by hexokinase. Glucose-6-phosphate (G6P) is then oxidised to 6-phospho-gluconate in the presence of oxidised nicotinamide adenine dinucleotide (NAD) in a reaction catalysed by glucose-6-phosphate dehydrogenase (G6PDH). During this oxidation, an equimolar amount of NAD is reduced to NADH. The consequent increase in absorbance at 340 nm is directly proportional to glucose concentration.