Bacterial Ssaining, viability/cytotoxicity assay kit for bacteria live and dead cells

Leverandør: Biotium
Ratings: Ingen bedømmelse

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30027.EA 3020 DKK
30027.
Bacterial Ssaining, viability/cytotoxicity assay kit for bacteria live and dead cells
Assays Cellulær Assays
The Bacteria Viability/Cytotoxicity Assay Kit provides two-colour fluorescence staining of both live bacteria (green) and dead bacteria (red) using two probes, DMAO and EthD-III.

  • Detect live and dead bacteria cells in a cell population simultaneously
  • Rapid (15 minute) dye loading and measure without washing
  • Perform viability and cytotoxicity assays at the same time
  • Analysis compatible with flow cytometers and fluorescence microscopes using popular settings for fluorescein and propidium iodide

DMAO is a green-fluorescent nucleic acid dye which stains both live and dead bacteria with intact and damaged cell membranes. EthD-III is a red-fluorescent nucleic acid dye that only stains dead bacteria with damaged cell membranes. With an appropriate mixture of DMAO and EthD-III, bacteria with intact cell membranes stain fluorescent green, whereas bacteria with damaged cell membranes stain fluorescent red. The assay principles are general and applicable to most bacteria types.

Leveringsinformation: Kit contains 2 vials DMAO, 5 mM (100 µl/vial) and 2 vials (150 µl/vial): Ethidium Homodimer-III (EthD-III), 2 mM (150 µl/vial).
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