Pierce™ immobilised TPCK trypsin is a serine endoprotease applicable to amino acid analysis and protein sequencing, mapping and structural studies. The immobilised form allows sample separation after treatment.
- Cleaves at carboxyl side of arginine and lysine residues
- Digestion conditions: pH 7,5 to 9,0, 37 °C
- Immobilised for ease of separation from cleavage products or affinity purification of trypsin inhibitors
Immobilised TPCK trypsin minimises autolysis, eliminates contamination of a sample with the protease and allows control of digestion by removing the trypsin. Immobilised trypsin is also more stable against heat-induced denaturation, resulting in longer maintenance of activity. Applications include removal of adherent cells from tissue culture flasks, preparing tryptic fragments for Edman degradation sequencing and for purifying soybean trypsin inhibitor.
Leveringsinformation: Kit contains immobilised TPCK trypsin, 2 ml of settled gel supplied as a 50% slurry containing glycerol, and 0,05% sodium azide as a preservative.