Pierce™ F(ab')2 Micro Preparation Kit enables efficient Fab generation from IgG.
- Enzyme free digestion products
- Enhanced yield and sample purity
- Spin format reduces sample processing time
- Flexible, protocols are included for multiple species and IgG subclasses, as well as sample size and concentration
Pierce™ F(ab')2 Micro Preparation Kit uses immobilised pepsin protease to selectively digest whole human and other IgG antibodies to make F(ab')2 fragments that retain antigen binding activity. The kit procedure is optimised for human, rabbit and mouse IgG. Pepsin-based digestion is effective for other species and subclasses of IgG, although the purification step requires that the antibody effectively binds to Protein A. Immobilised pepsin (beaded agarose resin) provides for control of the digestion reaction and complete removal of resulting antibody fragments from the proteolytic enzyme.
Leveringsinformation: Kit includes pepsin agarose (0,5 ml), F(ab')2 digestion buffer (55 ml), NAb protein A Plus spin columns, 0,2 ml (2 columns), PBS packs (each makes 500 ml) (2 packs), IgG elution buffer (50 ml), Zeba spin desalting columns, 7K MWCO, 0,5 ml (10 columns), spin columns, 0,8 ml (10 columns) and microcentrifuge tubes, 2 ml (30 tubes).
