Yeast β-galactosidase assay kit, Pierce™

Leverandør: Thermo Fisher Scientific
Ratings: Ingen bedømmelse

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PIER75768EA 2090 DKK
PIER75768
Yeast β-galactosidase assay kit, Pierce™
Assays Enzym Assays
Yeast β-Galactosidase Assay Kit is specially formulated for yeast cells and ideal for identifying protein interactions in vivo using two-hybrid systems.

  • Efficient lysis of yeast cells and a colorimetric detection system
  • Quantitative or qualitative assay for yeast colonies grown on solid media or in suspension
  • Colorimetric assay easily measured in a plate reader or spectrophotometer (420 nm)
  • Adaptable to 96-well microplates or tube assay formats
  • Perform homogeneous screening assays or measure activity secondarily after cell lysis

Yeast β-Galactosidase Assay Kit kit allows qualitative or quantitative determination of β-galactosidase activity in solution directly from colonies growing on solid medium. A portion of the colony is suspended in a mixture of Y-PER yeast protein extraction reagent and β-galactosidase assay buffer. After a brief incubation period, the solution turns yellow from the hydrolysis of o-nitrophenyl-β-D-galactopyranoside (ONPG) to o-nitrophenol (ONP) and galactose in a mildly alkaline solution. The assay becomes quantitative if calibrated to cell number by measuring the optical density at 660 nm.

Leveringsinformation: Kit contains Y-PER™ Yeast Protein Extraction Reagent, 25 ml; 2X β-Galactosidase Assay Buffer, 25 ml ; and 1 M Sodium Carbonate Stop Solution, 25 ml.
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