Hygromycin B ≥80%

Leverandør: MP Biomedicals
Ratings: Ingen bedømmelse

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ICNA0215751383
Hygromycin B ≥80%
Hygromycin B

Hygromycin B is an aminoglycoside antibiotic produced by the bacterium Streptomyces hygroscopicus that kills bacteria, fungi and higher eukaryotic cells by inhibiting protein synthesis.


Hygromycin has been used to select drug-resistant stable transfectants after transfer of the hygromycin phosphotransferase gene in a variety of cell culture applications. It is used in gene transfer experiments as a selection agent.


Mode of action: Inhibition of protein synthesis. It interferes with translocation of the 70S ribosome by inducing the misreading of the m-RNA template in prokaryotes and eukaryotes. It selectively penetrates cells that have been rendered permeable by virus infection.


Hygromycin B is provided as a caramel-coloured solution in water. It is not prepared under sterile conditions. If a sterile solution is desired, working solutions of Hygromycin B in aqueous media can be passed through a 0.2 micron filter before use.


Freely soluble in water, methanol, buffer solution and ethanol. Practically insoluble in less polar solvents.


Advarsel: Do not autoclave solutions of Hygromycin B.

Formel: C₂₀H₃₇N₃O₁₃
Molekylvægt: 527,5 g/mol
MDL Number: MFCD06795479
CAS-nummer: 31282-04-9

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Specifikationer Testresultater

Synonyms Antihelmycin; Hygromix-8
Typical Working Concentration For use as a selective agent, the suggested concentration range is 100- 800 µg/mL
Form Clear Golden Yellow Liquid
Storage Store at +4 °C.
pka 7.1 and 8.8(Lit.)
Specific Activity ~450,000 u/mL
Sterilization of Solutions Solutions should be sterilized by means of a 0.2 micron filter. Do not autoclave solutions.

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