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Anti-GAPDH Rabbit Polyclonal Antibody

Leverandør: Thermo Scientific
Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Suggested positive control: antigen standard for GAPDH (transient overexpression lysate). GAPDH is a 146 kDa tetramer composed of four 30-40 kDa subunits. Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) is a metabolic enzyme responsible for catalyzing one step in the glycolytic pathway, the reversible oxidative phosphorylation of glyceraldehyde 3-phosphate. Because GAPDH as a protein expressed in large amounts and which is required at all times for an important house keeping functions, levels of GAPDH mRNA are often used as standards in studies of mRNA expression. Increasingly, scientists are making use of specific antibodies to GAPDH as loading controls for western blotting experiments. Apart from a role in glycolysis, GAPDH may have other roles such as in the activation of transcription. GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease, and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease. Associations with actin and tubulin have also be reported. The protein may also have a role in the regulation of apoptosis, and interestingly migrates from the cytoplasm into the nucleus when cells become apoptotic.

Type: Primary
Antigen: GAPDH
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Rabbit
Isotype: IgG
Reactivity: Primates, Rat, Mouse, Human



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Specifikationer

Antigen symbol GAPDH
Antigen name Glyceraldehyde-3-phosphate dehydrogenase
Reactivity Primates, Human, Rat, Mouse
Conjugation Unconjugated
Clonality Polyclonal
Antibody type Primary
Host Rabbit
Isotype IgG
Western Blot Yes
ImmunoChemistry Yes
ImmunoFluorescence Yes
Flow Cytometry Yes
Antigen synonyms G3PD, GAPD, G3PDH, Glyceraldehyde-3-Phosphate Dehydrogenase
Gene ID 14433, 24383, 2597
Koncentration 0.2 mg/ml
Immunogen Residues between 150 and 200 of human Glyceraldehyde-3-Phosphate Dehydrogenase (GeneID 2597)
Purification Antigen affinity chromatography
Form Liquid
Storage Buffer TBS, 0.1% BSA, 0.09% sodium azide
Opbevaringstemperatur 4° C, do not freeze
Shipping Temperature Ice

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