qScript™ XLT cDNA SuperMix is a next generation tool for first-strand cDNA synthesis, providing a highly sensitive and easy to use solution for two-step RT-PCR and RT-qPCR. qScript XLT is an engineered M-MLV reverse transcriptase with reduced RNase H activity and improved activity and stability at higher temperatures. Combined with a precise mixture of reaction components, this SuperMix enables superior results over a wide dynamic range of input RNA, with up to 8-fold higher sensitivity than cDNA synthesis kits utilising an RNase H(+) reverse transcriptase (RT).
- Improved performance over traditional reverse transcriptases
- Broad linear dynamic range
- RNase H minus RT = higher yield = higher sensitivity
- Ability to reverse transcribe difficult sequences resulting in improved representation of problematic sequences and longer first-strand product
This 5X concentrated master mix provides all necessary components (except RNA template) for first-strand synthesis. The unique blend of oligo (dT) and random primers in the qScript XLT cDNA SuperMix captures unbiased representation of all RNA sequences into cDNA product (including the 5’-end, 3’-end, or central regions of long RNAs) and works exceptionally well with a wide variety of RNA templates. However, due to the relatively short average length of random-primed first-strand product, amplicon length for conventional RT-PCR applications should be limited to 1 kb or less.
Leveringsinformation: qScript™ XLT cDNA SuperMix includes 5X reaction buffer containing optimised concentrations of MgCl₂, dNTPs (dATP, dCTP, dGTP, dTTP), recombinant RNase inhibitor protein, qScript reverse transcriptase, random primers, oligo(dT) primer and stabilisers.