Cell culture media, Iscove's Modified Dulbecco's Medium (IMDM)Leverandør: Biowest
Ratings: Ingen bedømmelse
P0192-N1LEA 69.7 DKKP0192-N1L P0191-N10L L0190-500 P0191-N50L P0192-N10L L0192-500 L0191-500 P0191-N1L
Cell culture media, Iscove's Modified Dulbecco's Medium (IMDM)
In 1976, Guilbert and Iscove demonstrated that precursor cells of erythrocytes and macrophages could be cultured in a reduced-serum medium supplemented with albumin, transferrin, lecithin, and selenium. Iscove's medium is a modification of Dulbecco's Modified Eagle's Medium (DME) containing selenium, additional amino acids and vitamins, sodium pyruvate, HEPES buffer, and potassium nitrate instead of ferric nitrate. Further studies have demonstrated that Iscove's medium will support murine B lymphocytes, haemopoietic tissue from bone marrow, B-cells stimulated with lipopolysaccharide, T-lymphocytes, and a variety of hybrid cells.