SDS-PAGE clean up kit is designed to prepare samples for SDS-PAGE that are frequently difficult to analyse due to the presence of salts or low protein concentration.
- Prepares samples for SDS-PAGE that are otherwise difficult to analyse because of salts or low protein concentrations
- Quantitatively precipitate proteins while leaving interfering substances in solution
- Delivers quantitative yield in less than 2 hours
SDS-PAGE clean up kit uses a combination of a precipitant and co-precipitant to quantitatively precipitate the sample proteins while leaving interfering substances in solution. Detergents, salts, lipids, phenolics, and nucleic acids remain soluble while the proteins are pelleted by centrifugation. The precipitate is washed further to remove nonprotein contaminants and centrifuged again. The resultant pellet is resuspended, mixed with SDS-PAGE sample buffer, and heated. The sample is then ready for SDS-PAGE. The procedure can be completed in under 2 hours with quantitative yield.
Bestillingsoplysninger: Kit contains sufficient reagents for 50×100 μl samples: 15 ml Precipitant, 15 ml Co-precipitant, 50 ml Wash buffer, 250 μl Wash additive, 2 ml Buffer I, 500 μl Buffer II, Quick reference card and instructions.