Affinity chromatography column, HisPrep™ FF

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HisPrep™
28-9365-51EA 3730 DKK
28-9365-51
Affinity chromatography column, HisPrep™ FF
Kromatografikolonner
HisPrep™ FF 16/10 is a ready-to-use column, prepacked with precharged Ni Sepharose™ 6 Fast Flow which consists of 90 mm beads of highly cross-linked agarose, to which a chelating ligand has been immobilised. This chelating ligand is charged with Ni²⁺ ions, the first-choice metal ion for purifying most histidine-tagged proteins.

  • Compatible with a wide range of reducing agents, detergents, denaturants, and other additives
  • Negligible leakage of Ni²⁺
  • High binding capacity, approximately 40 mg/ml medium
  • Convenient and time-saving 20 ml prepacked HiPrep format

Ni Sepharose™ 6 Fast Flow consists of 90 m beads of highly cross-linked agarose, to which a chelating ligand has been immobilized. This chelating ligand is charged with Ni²⁺ ions, the first-choice metal ion for purifying most histidine-tagged proteins. The negligible leakage of Ni²⁺ ions ensures reliable capture of histidine-tagged proteins in repeated IMAC purifications.
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