Affinity chromatography column, HiTrap™ IgY purification HPLeverandør: Cytiva
Ratings: Ingen bedømmelse
17-5111-01EA 5120 DKK17-5111-01
Affinity chromatography column, HiTrap™ IgY purification HP
HiTrap IgY Purification HP is a 5 ml column prepacked with Sepharose High Performance for fast and easy purification of IgY from egg yolk.
- Each column binds IgY from a one-quarter portion of egg yolk (approx. 100 mg IgY). For greater capacity, several columns may be connected in series
- Packed with a thiophilic adsorption medium, the ligand, 2-mercaptopyridine is coupled to Sepharose High Performance
- Recovery and purity of IgY are excellent
- Can be operated with a liquid chromatography system such as ÄKTA design
- The top and bottom frits are manufactured from porous polyethylene
HiTrap IgY Purification HP is packed with a thiophilic adsorption medium, 2-mercaptopyridine coupled to Sepharose High Performance. Thiophilic adsorption is promoted by water-structuring salts. It has been suggested that the interaction of IgY and ligand results from combined electron donating and accepting action of the ligand or, alternatively, a mixed mode hydrophilic-hydrophobic interaction.
Leveringsinformation: Connectors for usage with different chromatography systems and other equipment are included.