Affinity chromatography columns, GSTrap™ FF

Leverandør: Cytiva
Ratings: Ingen bedømmelse

Samlet vurdering
Gennemsntilig vurdering

GSTrap™
17-5131-01EA 2434 DKK
17-5131-01 17-5130-01 17-5131-02 17-5130-02
Affinity chromatography columns, GSTrap™ FF
Kromatografikolonner
GSTrap FF columns are prepacked Glutathione Sepharose Fast Flow columns for fast, convenient, one-step purification of glutathione S-transferase (GST) tagged proteins.

  • Fast and simple one-step purification of GST-tagged proteins
  • Mild elution conditions preserving protein antigenicity and function
  • Easy scale-up by connecting the columns in series
  • Sepharose 4 Fast Flow provides good flow properties

GST-tagged proteins can be purified directly from pretreated bacterial lysates using GSTrap FF.GST-tagged proteins are eluted under mild, nondenaturing conditions using reduced glutathione.

The purification process preserves protein antigenicity and function. If desired, cleavage of the protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged proteins can be detected using colorimetric or immunological methods.

The resin, Glutathione Sepharose 4 Fast Flow, is also available as lab packages and is an excellent choice for scale-up.The columns can be operated with a syringe, peristaltic pump, or liquid chromatography system such as ÄKTA design or FPLC System.
Bestil nu

Learn more

About VWR

Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...

Læs mere About VWR