Affinity chromatography columns, HisTrap™ FF crude

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Histrap™
29-0486-31EA 334 DKK
29-0486-31 11-0004-58 17-5286-01
Affinity chromatography columns, HisTrap™ FF crude
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HisTrap™ FF crude columns offer the convenience of simple, one-step purification of histidine-tagged proteins directly from homogenized, unclarified cell lysate by immobilized metal ion affinity chromatography (IMAC). The columns are pre-packed with precharged Ni Sepharose™ 6 Fast Flow. Ni Sepharose™ 6 Fast Flow has low nickel ion (Ni²⁺) leakage and is compatible with a wide range of additives used in protein purification.

  • High binding capacity and low nickel ion leakage
  • Ensures reliable capture of histidine-tagged proteins in repeated IMAC purifications
  • Compatible with a wide range of reducing agents, detergents, denaturants and other additives
  • Simple manual operation with a syringe, pump or chromatography system such as ÄKTA design or FPLC system
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