HPLC Method Development, the Importance of Selectivity

Method development often begins, for the sake of practicality, with testing a C18 column. However, selectivity is a crucial parameter in liquid chromatography, which must be taken into account for a good method development. And selectivity is not only about testing other stationary phases, it is also about understanding how the column works to select the right one(s) to screen during our development. In this presentation, we will discuss the main interactions existing in LC, to better understand “what’s behind” the stationary phases, and in order to choose the right column from looking directly at the molecule(s) structure.