From Bench Top Automated Imaging To High Throughput Screening: High Content Analysis of 2-D & 3-D Cellular Models

Friday, 29th May, 2020
11:00 to 12:00 CEST (Berlin, Paris, Madrid)

Replay

There is an increased need for expanding the variety and complexity of cell-based assays for biologic research and drug discovery across a range of areas, including virology, toxicology and cancer research. Increasingly, three-dimensional (3-D) cultures are used for assay development and phenotypic screening for a range of cellular models, including CNS, tumour micro-tissues and organoids. Combining these 2-D and 3-D models with 'live' cell assays, allows monitoring of cell responses in real time, and provides important insights about compound treatment effects, biological complexity, and physiological relevance of assay results. Meanwhile, a range of high content imaging systems are now available, allowing researchers to obtain quantitative, relevant data from their samples faster than ever before. 
 
High content imaging and analysis with the ImageXpress® Micro Confocal and the ImageXpress Pico enables quantitative characterisation of 2-D and 3-D cellular models. State-of-the-art optics and powerful analysis tools generate phenotypic data from complex models in a high throughput automated manner, increasing throughput and streamlining your workflow. 
 
Key highlights:

  • Use high content analysis to analyse multiple parameters across high throughput compound screens quickly and efficiently 
  • Obtain quantitative, actionable data across a vast range of applications, from virus research and toxicology screening to 'early' stage drug discovery
  • Overcome challenges in automating 3-D organoid imaging with robust and flexible autofocusing methods, and targeted imaging workflows
  • Characterise compound effects on tumour spheroid phenotypes and volumetric changes in 3-D cellular content with 3-D image analysis algorithms